Makoto Kawamukai. Biosynthesis of coenzyme Q in eukryotes. Biosci. Biotechnol. Biochem. 80: 23-33 (2016)


戒能智宏、川向誠、第4章 生合 成と欠損症、「コエンザイムQ10基礎と応用」(山本順寛編)丸善 2015

Daisuke Moriyama, Kouji Hosono, Makoto Fujii, Motohisa Washida, Hirokazu Nanba, Tomohiro Kaino, Makoto Kawamukai. Production of CoQ10 in fission yeast by expression of genes responsible for CoQ10 biosynthesis. Biosci. Biotechnol. Biochem. 79(6):1026-1033 (2015)


Murai M, Matsunobu K, Kudo S, Ifuku K, Kawamukai M, Miyoshi H. Identification of the Binding Site of the Quinone-Head Group in Mitochondrial Coq10 by Photoaffinity Labeling. Biochemistry 53:3359-4003 (2014)

Kazuhiro Hayashi, Yuki Ogiyama, Kazumasa Yokomi, Tsuyoshi Nakagawa, Tomohiro Kaino, Makoto Kawamukai. Functional conservation of coenzyme Q biosynthetic genes among yeasts, plants, and humans. PLoS ONE 9(6)e99038 (2014)

川向誠 戒能智宏 太田明徳 福 田良一 脂質の代謝 分担執筆 酵母の生命科学と生物工学(原島 高木編)化学同人 2013

Tatsuro Nishida, Tomohiro Kaino, Ryo Ikarashi, Daisuke Nakata, Keiji Terao, Masahiro Ando, Hiroo Hamaguchi, Makoto Kawamukai, Tatsuyuki Yamamoto. The effect of coenzyme Q10 included by gamma-cyclodextrin on the growth of fission yeast studied by microscope Raman spectroscopy. J. Molecular Structure  375-381(2013)

Mi-Suk Seo, Sakiko Takahashi, Koh-ichi Kadowaki, Makoto Kawamukai, Manabu Takahara, Tadashi Takamizo. Expression of CoQ10-producing ddsA transgene by efficient Agrobacterium-mediated transformaion in Panicum meyerianum. Plant Cell Tiss Organ Cult 107:325-332(2011)

川向 誠、コエンザイムQ10生産微 生物の開発、生物工学会誌、89:323-325(2011)

Nobuhiko Mukai, Kazuo Masaki Tsutomu Fujii, Makoto Kawamukai, Haruyuki Iefuji. PAD1 and FDC1 are essential for the decarboxylation of phenylacrylic acids in Saccharomyces cerevisiae. J. Biosci. Bioengin. 109:564-569 (2010).

Tie-Zhong Cui, Tomohiro Kaino, Makoto Kawamukai. A subunit of decaprenyl diphosphate synthase stabilizes octaprenyl diphosphate synthase in Escherichia coli by forming a high-molecular weight complex. FEBS Lett 584, 652-656 (2010)

Sakiko Takahashi, Toshikazu Ohtani, Hikaru Satoh, Yasunori Nakamura, Makoto Kawamukai, Koh-Ichi Kadowaki. Development of Coenzyme Q10-enriched rice using sugary and shrunken mutants. Biosci. Biotechnol. Biochem. 74, 182-184 (2010)


Sakiko Takahashi, Toshikazu Ohtani, Shuichi Iida, Yoshihiro Sunohara, Kei Matsushita, Hideo Maeda, Yoshitaka Tanetani, Kiyoshi Kawai, Makoto Kawamukai, Koh-Ichi Kadowaki. Development of CoQ10-riched rice from giant embryo lines. Breeding Science 59:321-326 (2009)
Makoto Kawamukai. Biosynthesis and bioproduction of coenzyme Q10 by yeasts and other organisms. Biotechnol. Appl. Biochem. 53:217-226 (2009)

Tie-Zhong Cui, Makoto Kawamukai. Coq10, a mitochondrial coenzyme Q binding protein, is required for proper respiration in Schizosaccharomyce pombe FEBS J. 276:748-759 (2009)

Makoto Kawamukai Biosynthesis and bioproduction of coenzyme Q10 by yeasts and other organisms.Biotechnol Appl Biochem. 53:217-426  (2009)


Mei Zhang, Shusoh Wakitani, Kazuhiro Hayashi, Risa Miki, Makoto Kawamukai. High production of sulfide in coenzyme Q deficient fission yeast Biofactors 32, 91-98 (2008)


Risa Miki, Ryoichi Saiki, Yoshihisa Ozoe, Makoto Kawamukai. Comparison of a coq7 deletion mutant with other respiration-defective mutants in fission yeast. FEBS J. 275:5309-5324(2008)

Ryoichi Saiki, Adam L. Lunceford, Yuchen Shi, Beth Marbois, Rhonda King, Justin Pachuski, Makoto Kawamukai, David L. Gasser, and Catherine F Clarke, Coenzyme Q10 supplementation rescues renal disease in Pdss2 kd/kd mice with mutations in prenyl diphosphate synthase subunit2. Am J Physiol Renal Physiol. 295 F1534-1544 (2008)

Mei Zhang, Jun Luo, Yuki Ogiyama, Ryoichi Saiki, Makoto Kawamukai. Heteromer formation of a long-chain prenyl diphosphate synthase from fission yeast Dps1 and budding yeast Coq1. FEBS J. 275:3653-3668 (2008)


川向 誠 コエンザイムQ10の多彩な生理機能と新生産法の開拓、バイオサイエンスとインダストリー65,463-465(2007)

Yanfang Ye, Makoto Fujii, Aiko Hirata, Makoto Kawamukai. Chikashi Shimoda, Taro Nakamura. Geranylgeranyl diphosphate synthase in fission yeast is a heteromer of farnesyl diphosphate synthase (FSP), Fps1 and FPS-like protein , Spo9, essential fo sporulation. Mol. Biol. Cell 18,3568-3581(2007) 

Ferella M, Montalvetti A, Rohloff P, Miranda K, Fang J, Reina S, Kawamukai M, Bua J, Nilsson D, Pravia C, Katzin A, Cassera MB, Aslund L, Andersson B, Docampo R, Bontempi EJ.   A solanesyl diphosphate synthase localizes in glycosomes of Trypanosoma cruzi. J Biol Chem. 281,39339-39348 (2006)

Sakiko Takahashi, Yuki Ogiyama, Hiroaki Kusano, Hiroaki Shimada, Makoto Kawamukai, Koh-ichi Kadowaki. Metabolic engineering of Coenzyme Q by modification of isoprenoid side chain in plant. FEBS Lett. 580:955-959 (2006)

Ryoichi Saiki, Ai Nagata,Tomohiro Kainou, Hideyuki Matsuda, and Makoto Kawamukai.Characterization of solanesyl and decaprenyl diphosphate synthases in mice and humans. FEBS J. 56:5606-5622 (2005)

Park YC, Kim SJ, Choi JH, Lee WH, Park KM, Kawamukai M, Ryu YW, Seo JH.Batch and fed-batch production of coenzyme Q(10) in recombinant Escherichia coli containing the decaprenyl diphosphate synthase gene from Gluconobacter suboxydans.Appl Microbiol Biotechnol. 67, 192-196 (2005)

Luo Jun,Ryoichi Saiki, Kei Kawamura, Tsuyoshi Nakagawa, and Makoto Kawamukai. Identification and subcellular localization of two solanesyl diphosphate synthases from Arabidopsis thaliana.Plant Cell Physiol. 45:1882-1888.(2004)

Surang Engprasert, Futoshi Taura, Makoto Kawamukai, Yukihiro Shoyama, Molecular cloning and functional expression of geranylgeranyl pyrophosphate synthase from Coleus forskohlii Briq. BMC Plant Biol. on line (2004)

Park YC, Kim SJ, Choi JH, Lee WH, Park KM, Kawamukai M, Ryu YW, Seo JH.Batch and fed-batch production of coenzyme Q(10) in recombinant Escherichia coli containing the decaprenyl diphosphate synthase gene from Gluconobacter suboxydans.Appl Microbiol Biotechnol. (published on line)

Kazunori Okada, Kazuaki Ohara, Kazufumi Yazaki, Kouhei Nozaki, Naonori Uchida, Makoto Kawamukai, Hideaki Nojiri and Hisakazu Yamane, The AtPPT1 gene encoding 4-hydroxybenzoate polyprenyl diphosphate transferase in ubiquinone biosynthesis is required for embryo development in Arabidopsis thaliana.Plant Mol. Biol.55:567-577 (2004)

Ryoichi Saiki, Yuki Ogiyama, Tomohiro Kainou, Tomoko Nishi, Hideyuki Matsuda, and Makoto Kawamukai. Pleiotropic phenotypes of fission yeast defective in ubiquinone-10 production. A study from the abc1Sp (coq8Sp) mutant. Biofactors 18:229-235 (2003)

Ryoichi Saiki, Ai Nagata, Naonori Uchida, Tomohiro Kainou, Hideyuki Matsuda, and Makoto Kawamukai. Fission yeast
decaprenyl diphosphate synthase consists of Dps1 and the newly characterized Dlp1 protein in a novel heterotetrameric
structure. Eur. J. Biochem. 270:4113-4121 (2003)

Makoto Kawamukai. Biosynthesis, bioproduction and novel roles of ubiquinone. J. Biosci. Bioeng. 94, 511-517(2002)

川向 誠、ユビキノンの生合成と新しい 生理的機能、化学と生物、40(5);172-178 (2002)

川向 誠、ユビキノン発酵「発酵ハンドブック」共立出版 p406-407, (2001)

Tomohiro Kainou, Kazunori Okada, Kengo Suzuki, Tsuyoshi Nakagwa, Hideyuki Matsuda and Makoto Kawamukai. Dimer formation of octaprenyl diphosphate synthase (IspB) is essential for chain length determination of ubiquinone. J. Biol. Chem. 276 (11), 7876-7883, (2001)

Naonori Uchida, Kengo Suzuki, Ryoichi Saiki, Tomohiro Kainou, Katsunori Tanaka, Hideyuki Matsuda and Makoto Kawamukai.ハ Phenotypes of fission yeast defective in ubiquinone production due to disruption of the gene for p-hydroxybenzoate polyprenyl diphosphate tranferase. J. Bacteriol. 182: 6933-6939 (2000)

Kazunori Okada, Takeshi Saito, Tsuyoshi Nakagwa, Makoto Kawamukai and Yuji Kamiya. Five geranylgeranyl diphosphate synthases expressed in different organs are localized into three subcellular compartments in Arabidopsis. Plant Physiol. 122:1045-1056 (2000)

Tomohiro Kainou, Kei Kawamura, Katsunori Tanaka, Hideyuki Matsuda and Makoto Kawamukai. Identification of the GGPS1 genes encoding geranylgeranyl diphosphate synthases from mouse and human. Biochim. Biophys.Acta. 1437:333-340 (1999)

川向 誠、ユビキノンの生合成、機能に関する最近の展開、生化学、70:1344-1349 (1998)

Kazunori Okada, Tomohiro Kainou, Hideyuki Matsuda and Makoto Kawamukai. Biological significance of the side chain length of ubiquinone in Saccharomyces cerevisiae. FEBS lett. 431:241-244 (1998)

Kazunori Okada, Tomohiro Kainou, Katsunori Tanaka, Tsuyoshi Nakagawa, Hideyuki Matsuda and Makoto Kawamukai. Molecular cloning and mutational analysis of the ddsA gene encoding decaprenyl diphosphate synthase from Gluconobacter suboxydans. Eur. J. Biochem. 255(1): 52-59 (1998)

Kazunori Okada, Yasuhiro Kamiya, XuFen Zhu, Kengo Suzuki, Katsunori Tanaka, Tsuyoshi Nakagawa, Hideyuki Matsuda and Makoto Kawamukai. Cloning of the sdsA gene encoding solanesyl diphosphate synthase from Rhodobacter capsulatus and its functional expression in Escherichia coli and Saccharomyces cerevisiae. J. Bacteriol. 179:5992-5998 (1997)

Kazunori Okada, Masaru Minehira, XuFen Zhu, Kengo Suzuki, Tsuyoshi Nakagawa, Hideyuki Matsuda and Makoto Kawamukai. The ispB gene encoding octaprenyl diphosphate synthase is essential for growth of Escherichia coli. J. Bacteriol 179:3058-3060 (1997)

XuFen Zhu, Kengo Suzuki, Takeshi Saito, Kazunori Okada, Katsunori Tanaka, Tsuyoshi Nakagawa, Hideyuki Matsuda and Makoto Kawamukai. Geranyl geranyl pyrophosphate synthase encoded by the newly isolated gene GGPS6 from Arabidopsis thaliana is localized in mitochondria. Plant Mol Biol. 35:331-341   (1997)

Kengo Suzuki, Kazunori Okada, Yasuhiro Kamiya, XuFen Zhu, Tsuyoshi Nakagawa, Makoto Kawamukai and Hideyuki Matsuda. Analysis of the decaprenyl diphoshate synthase (dps) gene in fission yeast suggests a role of ubiquinone as an antioxidant. J. Biochem. 121:496-505 (1997)

XuFen Zhu, Kengo Suzuki, Kazunori Okada, Katsunori Tanaka, Tsuyoshi Nakagawa, Makoto Kawamukai and Hideyuki Matsuda. Cloning and functional expression of a novel geranylgeranyl pyrophosphate synthase gene from Arabidopsis thaliana in Escherichia coli. Plant Cell Physiol. 38:357-361 (1997)

Kazunori Okada, Kengo Suzuki, Yasuhiro Kamiya, XuFen Zhu, Shingo Fujisakai, Yukinobu Nishimura, Tokuzo Nishino, Tsuyoshi Nakagawa, Makoto Kawamukai, Hideyuki Matsuda. Polyprenyl diphosphate synthase essentially defines the length of the side chain of ubiquinone. Biochim. Biophys.Acta. 1302/3:217-223 (1996)

川向 誠、ユビキノンの種類を決定する因子。バイオサイエンスとインダストリー   54:45-47. (1996)

XuFen Zhu, Mihoko Yuasa, Kazunori Okada, Kengo Suzuki, Tsuyoshi Nakagawa, Makoto Kawamukai and Hideyuki Matsuda. Production of ubiquinone in Escherichia coli by expression of various genes responsible for ubiquinone biosynthesis. J. Ferment. Bioeng. 79(5):493-495. (1995).

Kengo Suzuki, Minoru Ueda, Mihoko Yuasa, Tsuyoshi Nakagawa, Makoto Kawamukai, and Hideyuki Matsuda. Evidence that E. coli ubiA product is a functional homolog of yeast COQ2 and the regulation of the ubiA gene expression. Biosci. Biotech. Biochem. 58(10), 1814-1819. (1994).

Ken-ichi Asai, Shingo Fujisaki, Yukinobu Nishimura, Tokuzo Nishino, Kazunori Okada, Tsuyoshi Nakagawa, Makoto Kawamukai and Hideyuki Matsuda. The identification of Escherichia coli ispB (cel) gene encoding the octaprenyl diphosphate synthase. Biochem. Biophys. Res. Commun. 202:340-345. (1994).